ELISA Detection of Kudoa septempunctata in Raw Paralichthys olivaceus (Olive Flounder) using a Chicken Anti-Kudoa Antiserum
نویسندگان
چکیده
منابع مشابه
ELISA detection of Kudoa septempunctata in raw Paralichthys olivaceus (olive flounder) using a chicken anti-Kudoa antiserum.
Kudoa septempunctata is the causative agent of a foodborne disease associated with the consumption of raw Paralichthys olivaceus (olive flounder). Chickens were used to establish specific antibodies against K. septempunctata spores. A specific antiserum, CS#3, raised against sonicated spores, also recognized intact spores. The CS#3 antiserum showed high titers for sonicated and intact K. septem...
متن کاملNew validated rapid screening methods for identifying Kudoa septempunctata in olive flounder (Paralichthys olivaceus).
Kudoa septempunctata is a newly identified causative agent of foodborne diseases associated with consuming raw olive flounder. Qualitative PCR and quantitative real-time PCR have been used as notification methods to identify K. septempunctata in Japan. However, these methods require expensive equipment and are time-consuming (2-3 h for screening). To address these problems, in this study, we de...
متن کاملLectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)
The localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-acetylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acety...
متن کاملEvaluation of the inflammatory response to Kudoa septempunctata genotype ST3 isolated from olive flounder (Paralichthys olivaceus) in Caco-2 cells
Kudoa septempunctata (Myxosporea, Multivalvulida) is a parasite of the trunk muscle of cultured olive flounder (Paralichthys olivaceus). We investigated whether K. septempunctata genotype ST3 spores induce cell damage and the secretion of inflammatory mediators in Caco-2 cells, which exhibit characteristics similar to human intestinal epithelial cells. Purified K. septempunctata spores were hea...
متن کاملInter-laboratory validation and applications of quantitative real-time PCR for the detection of Kudoa septempunctata in olive flounder (Paralichthys olivaceus).
Kudoa septempunctata, a myxosporean parasite, was recently identified as the causative agent of food poisoning resulting from the consumption of raw olive flounder (Paralichthys olivaceus). A single blind inter-laboratory study, involving 5 laboratories, was conducted to validate a quantitative real-time PCR assay for the detection of the parasite. We obtained relatively constant values for log...
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ژورنال
عنوان ژورنال: Biocontrol Science
سال: 2013
ISSN: 1342-4815,1884-0205
DOI: 10.4265/bio.18.193